The rRNA epitranscriptome and myonuclear SNORD landscape in skeletal muscle fibers contributes to ribosome heterogeneity and is altered by a hypertrophic stimulus
Minying Cui, Paulo R. Jannig, Maral Halladjian, Vandré C. Figueiredo, Yuan Wen, Ivan J. Vechetti, Nicolai Krogh, Baptiste Jude, Sebastian Edman, Johanna T. Lanner, John J. McCarthy, Kevin A. Murach, Thomas Sejersen, Henrik Nielsen, Ferdinand von Walden
Abstract
Ribosomal RNAs (rRNAs) are posttranscriptionally modified by 2′ O-methyl (2′- O-Me). This study applied RiboMeth-seq (RMS) to detect changes in 2′- O-Me levels during skeletal muscle hypertrophy, uncovering transient diversification of the ribosome pool in skeletal muscle fibers. This work implies a role for ribosome heterogeneity in skeletal muscle growth and adaptation.
Topics & Concepts
Skeletal muscleRibosomeRibosomal RNAMuscle hypertrophyBiologyCell biologyChemistryRNABiochemistryAnatomyEndocrinologyGeneRNA modifications and cancerRNA and protein synthesis mechanismsRNA Research and Splicing