Directed Signaling Cascades in Monodisperse Artificial Eukaryotic Cells
Sunidhi C. Shetty, Naresh Yandrapalli, Kerstin Pinkwart, Dorothee Krafft, Tanja Vidaković‐Koch, Ivan Ivanov, Tom Robinson
Abstract
the reconstitution of size-selective membrane pores. Therefore, the signal transduction, which is triggered externally, follows a specific spatial pathway between the compartments. We use this platform to study the effects of enzyme cascade compartmentalization by direct analytical comparison between bulk, one-, two-, and three-compartment systems. This microfluidic strategy to construct complex hierarchical structures is not only suitable to study compartmentalization effects on biochemical reactions but is also applicable for developing advanced drug delivery systems as well as minimal cells in the field of bottom-up synthetic biology.