Single cell-asymmetrical flow field-flow fractionation/ICP-time of flight-mass spectrometry (sc-AF4/ICP-ToF-MS): an efficient alternative for the cleaning and multielemental analysis of individual cells
Michail Ioannis Chronakis, Marcus von der Au, Björn Meermann
Abstract
A new approach relying on AF4 for gentle on-line removal of the ionic background from cells during single cell-ICP-ToF-MS analysis was developed. In a first proof-of-concept study the new approach was applied for the analysis of yeast cells.
Topics & Concepts
Mass spectrometryChemistryFractionationChromatographyAnalytical Chemistry (journal)Inductively coupled plasma mass spectrometryFlow (mathematics)Field flow fractionationMechanicsPhysicsField-Flow Fractionation Techniquesthermodynamics and calorimetric analyses