Thermally-modulated cell separation columns using a thermoresponsive block copolymer brush as a packing material for the purification of mesenchymal stem cells
Kenichi Nagase, Goro Edatsune, Yuki Nagata, Junnosuke Matsuda, Daiju Ichikawa, Sota Yamada, Yutaka Hattori, Hideko Kanazawa
Abstract
a temperature change from 37 °C to 4 °C. Additionally, the cellular activity of the MSCs was retained throughout the column separation step. Overall, the obtained results show that the developed column is useful for MSC separation without cell modification and cellular activity reduction.
Topics & Concepts
CopolymerAdsorptionElutionChemistryMesenchymal stem cellChromatographyPolymerizationCationic polymerizationChemical engineeringColumn chromatographyPolymer chemistryMaterials sciencePolymerOrganic chemistryCell biologyBiologyEngineeringElectrospun Nanofibers in Biomedical ApplicationsHydrogels: synthesis, properties, applicationsBone Tissue Engineering Materials