The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
Siyu Liu, Jun‐Jie Wu, Zhong‐Hua Chen, Ming‐Li Zou, Ying-ying Teng, Kai‐Wen Zhang, Yueyue Li, Dang-yang Guo, Feng‐Lai Yuan
Abstract
Purpose: To systematically analyze the overall m 6 A modification pattern in hyperplastic scars (HS). Methods: The m 6 A modification patterns in HS and normal skin (NS) tissues were described by m 6 A sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The m 6 A-related RNA was immunoprecipitated and verified by real-time quantitative PCR. Results: The appearance of 14,791 new m 6 A peaks in the HS sample was accompanied by the disappearance of 7,835 peaks. The unique m 6 A-related genes in HS were thus associated with fibrosis-related pathways. We identified the differentially expressed mRNA transcripts in HS samples with hyper-methylated or hypo-methylated m 6 A peaks. Conclusion: This study is the first to map the m 6 A transcriptome of human HS, which may help clarify the possible mechanism of m 6 A-mediated gene expression regulation.