Rapid room-temperature synthesis of biocompatible metal–organic framework for enzyme immobilization with improved stability and on-demand release
Yilun Weng, Penghui Yan, Baode Sun, Andria Wan, Jiakang You, Xin Xu, Zeyu Lu, G. A. Stewart, Xiaojing Chen, Hao Song, Chun‐Xia Zhao
Abstract
Enzyme immobilization within metal–organic frameworks (MOFs) addresses the inherent fragility of enzymes, playing a crucial role across diverse industries by improving efficiency and lowering economic costs. While the application of MOFs in the food and pharmaceutical industries is constrained by toxicity concerns, MIL-88A(Fe) emerges as an ideal candidate due to its non-toxicity and biocompatibility. However, the release of encapsulated enzymes is significantly hampered, reducing their bioactivity. Herein, we present a safe and simple platform for creating enzyme@MIL-88A, which provides enzyme stabilization and controlled release. The thermal stabilization of a spectrum of enzymes (phytase, xylanase, amylase, mannanase, and glucanase) is achieved, elevating their endurance threshold to 95 °C. Furthermore, the controlled on-demand release of the encapsulated enzymes at target sites is accomplished by adjusting defects in enzyme@MIL-88A composites via an acid modulation approach, while preserving enzyme activity. This approach has improved the amount of enzyme released from 10 % to 99.7 %. To the best of our knowledge, this is the first time enzyme@MIL-88A has been synthesized rapidly under mild conditions for enzyme stabilization and controlled release. Our method offers a universal platform for stabilizing vulnerable biomaterials and the controlled delivery of biological macromolecules.