A vacuum-assisted, highly parallelized microfluidic array for performing multi-step digital assays
Jiumei Hu, Liben Chen, Pengfei Zhang, Kuangwen Hsieh, Hui Li, Samuel Yang, Tza‐Huei Wang
Abstract
using a three-step loading approach and achieved accurate single-cell quantification. Taken together, we have demonstrated that our vacuum-driven microfluidic array is capable of multi-step sample digitalization at high throughput for single-molecule and single-cell analyses.
Topics & Concepts
MicrofluidicsBiochipReagentThroughputMaterials scienceOne-StepNanotechnologySingle-cell analysisDigital polymerase chain reactionComputer hardwareChemistryCellComputer scienceEngineeringChemical engineeringPhysical chemistryTelecommunicationsWirelessBiochemistryPolymerase chain reactionGeneMicrofluidic and Capillary Electrophoresis ApplicationsMicrofluidic and Bio-sensing TechnologiesElectrowetting and Microfluidic Technologies