piggyBac system to co-express NKG2D CAR and IL-15 to augment the in vivo persistence and anti-AML activity of human peripheral blood NK cells
Zhicheng Du, Yu Yang Ng, Shijun Zha, Shu Wang
Abstract
tumor control and significant prolongation of mouse survival in the KG-1 AML model. Collectively, our findings demonstrate the ectopic expression of IL-15 as an important means to improve the antileukemic activity of NKG2D CAR-NK cells. Our study further illustrates the feasibility of using the piggyBac non-viral platform as an efficient and cost-effective way for CAR-NK cell manufacturing.
Topics & Concepts
AugmentNKG2DPeripheral bloodPersistence (discontinuity)In vivoPeripheralBiologyImmunologyMedicineIn vitroCytotoxicityInternal medicineEngineeringBiochemistryGeotechnical engineeringBiotechnologyPhilosophyLinguisticsImmune Cell Function and InteractionCAR-T cell therapy researchTransgenic Plants and Applications