Cu-HKUST-1 and Hydroxyapatite–The Interface of Two Worlds toward the Design of Functional Materials Dedicated to Bone Tissue Regeneration
Marzena Fandzloch, Weronika Bodylska, Joanna Trzcińska-Wencel, Patrycja Golińska, Katarzyna Roszek, Joanna Wı́sniewska, Michał Bartmański, Agnieszka Lewińska, Anna Jaromin
Abstract
High Resolution Image Download MS PowerPoint Slide A novel composite based on biocompatible hydroxyapatite (HA) nanoparticles and Cu-HKUST-1 ( [email protected] ) has been prepared following a layer-by-layer strategy. Cu-HKUST-1 was carefully selected from a group of four Cu-based metal–organic frameworks as the material with the most promising antimicrobial activity. The formation of a colloidal Cu-HKUST-1 layer on HA nanoparticles was confirmed by various techniques, e.g., infrared spectroscopy, powder X-ray diffraction, N 2 sorption, transmission electron microscopy imaging, electron paramagnetic resonance, and X-ray absorption spectroscopy. Importantly, such a Cu-HKUST-1 layer significantly improved the nanomechanical properties of the composite, with Young’s modulus equal to that of human cortical bone (13.76 GPa). At the same time, [email protected] has maintained the negative zeta potential (−16.3 mV in pH 7.4) and revealed biocompatibility toward human dermal fibroblasts up to a concentration of 1000 μg/mL, without inducing ex vivo hemolysis. Chemical stability studies of the composite over 21 days in a buffer-simulated physiological fluid allowed a detailed understanding of the transformations that the [email protected] undergoes over time. Finally, it has been confirmed that the Cu-HKUST-1 layer provides antibacterial properties to HA, and the synergism reached in this way makes it promising for bone tissue regeneration.