Atomic and Specificity Details of Mucin 1 <i>O</i>-Glycosylation Process by Multiple Polypeptide GalNAc-Transferase Isoforms Unveiled by NMR and Molecular Modeling
Helena Coelho, Matilde de las Rivas, Ana Sofia Grosso, Ana Diniz, Cátia O. Soares, Rodrigo A. Francisco, Jorge S. Dias, Ismael Compañón, Lingbo Sun, Yoshiki Narimatsu, Sergey Y. Vakhrushev, Henrik Clausen, Eurico J. Cabrita, Jesús Jiménez‐Barbero, Francisco Corzana, Ramón Hurtado‐Guerrero, Filipa Marcelo
Abstract
, which highlights the relevance of GalNAc-T4 in the glycosylation of this epitope. Finally, the NMR methodology established herein can be extended to other glycosyltransferases, such as C1GalT1 and ST6GalNAc-I, to determine the specificity toward complex mucin acceptor substrates.
Topics & Concepts
GlycosylationGene isoformTandem repeatGlycanBiochemistryChemistryLectinTransferaseMucinBiologyGlycoproteinEnzymeGeneGenomeGlycosylation and Glycoproteins ResearchCarbohydrate Chemistry and SynthesisGalectins and Cancer Biology