Porcine Epidemic Diarrhea Virus nsp7 Inhibits MDA5 Dephosphorylation to Antagonize Type I Interferon Production
Jian-Song Zhang, Puxian Fang, Jie Ren, Sijin Xia, Huichang Zhang, Xuerui Zhu, Tong Ding, Shaobo Xiao, Liurong Fang
Abstract
, combines with nsp8 and nsp12 to form the viral replication and transcription complex that is indispensable for viral replication. However, the function of nsp7 in the infection and pathogenesis of coronaviruses remains largely unknown. Our present study demonstrates that PEDV nsp7 specifically competes with PP1 for binding MDA5 and impedes the PP1-mediated dephosphorylation of MDA5 at S828, thereby blocking MDA5-mediated IFN production, revealing the complex mechanism utilized by PEDV nsp7 to efficiently escape host innate immunity.
Topics & Concepts
Porcine epidemic diarrhea virusVirologyInterferonCoronavirusMDA5BiologyDephosphorylationIRF3VirusPhosphorylationTranscription factorCell biologyGenePhosphataseRNAGeneticsMedicineCoronavirus disease 2019 (COVID-19)PathologyInfectious disease (medical specialty)DiseaseRNA interferenceAnimal Virus Infections Studiesinterferon and immune responsesVirus-based gene therapy research