Efficient <i>in vitro</i> and <i>in vivo</i> transfection of self-amplifying mRNA with linear poly(propylenimine) and poly(ethylenimine-propylenimine) random copolymers as non-viral carriers
Lisa Opsomer, Somdeb Jana, Ine Mertens, Xiaole Cui, Richard Hoogenboom, Niek N. Sanders
Abstract
SaRNA was formulated with six different polymer carriers to generate saRNA-polyplexes. After screening for transfection efficiency, compared to a lipid-based standard, the best performing ones were characterized in detail and administered to mice.
Topics & Concepts
In vivoTransfectionIn vitroCopolymerMaterials scienceMessenger RNABiophysicsMolecular biologyPolymerChemistryBiochemistryBiologyGeneBiotechnologyComposite materialRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesBacteriophages and microbial interactions