Fabrication of gentamicin loaded Col-I/HA multilayers modified titanium coatings for prevention of implant infection
Le Ma, Jiajia Zong, Xiaowei Xun, Xiaoming Hu, Zejing Chen, Quanchao Zhang, Mengxia Peng, Botao Song, Haiyong Ao
Abstract
In this study, gentamicin loaded collagen I/hyaluronic acid multilayers modified titanium coating (TC-AA(C/H) 6 -G) was fabricated via a layer-by-layer (LBL) covalent immobilization method. The drug releasing properties of collagen I/Hyaluronic acid (Col-I/HA) multilayers and the effect of loaded gentamicin on the antibacterial properties and cytocompatibility of modified TC were investigated. The gentamicin release assay indicated that the Col-I/HA multilayers modified TC exhibited agreeable drug-loading amount (537.22 ± 29.66 µg of gentamicin) and controlled-release performance (240 h of sustained release time). TC-AA(C/H) 6 -G revealed satisfactory antibacterial activity and inhibited the colonization and biofilm formation of S. aureus . Fortunately, the functions of hMSCs on TC-AA(C/H) 6 -G did not affected by the loaded gentamicin, and TC-AA(C/H) 6 -G could improve the adhesion, proliferation and osteogenic differentiation of cells, as well as TC-AA(C/H) 6 . In vivo animal study indicated that TC-AA(C/H) 6 -G could effectively control intramedullary cavity infection caused by S. aureus and prevent bone destruction.