Efforts toward Further Integration of an Unnatural Base Pair into the Biology of a Semisynthetic Organism
Koji Hashimoto, Emil C. Fischer, Floyd E. Romesberg
Abstract
We have developed semisynthetic organisms (SSOs) that by virtue of a family of synthetic, unnatural base pairs (UBPs), store and retrieve increased information. To date, transcription in the SSOs has relied on heterologous expression of the RNA polymerase from T7 bacteriophage; here, we explore placing transcription under the control of the endogenous host multisubunit RNA polymerase. The results demonstrate that the E. coli RNA polymerase is able to transcribe DNA containing a UBP and that with the most optimal UBP identified to date it should be possible to select for increased uptake of unnatural triphosphates. These advances should facilitate the creation of next generation SSOs.
Topics & Concepts
ChemistryTranscription (linguistics)PolymeraseT7 RNA polymeraseHeterologousRNA polymeraseOrganismDNARNAComputational biologyRNA polymerase IIBase pairBacteriophageCell biologyBiochemistryGeneticsGeneGene expressionBiologyEscherichia coliPromoterLinguisticsPhilosophyRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniquesBacteriophages and microbial interactions