A mitochondria-targeting fluorescent probe for the dual-emission fluorescence-enhanced detection of hydrogen sulfide and turn-on detection of hydrazine
Yu-Man Tian, Shuangshuang Liu, Wei-Na Wu, Xiao‐Lei Zhao, Yuan Wang, Yun-Chang Fan, Zhi-Hong Xu, Tony D. James
Abstract
A fluorescent probe (probe 1) with selectivity and sensitivity for H2S and N2H4 has been developed. Probe 1 exhibits dual-excitation and dual-emission fluorescence response to H2S and fluorescence enhancement toward N2H4 with distinct naked-eye color changes. Moreover, probe 1 displayed low detection limits for H2S and N2H4 of 0.90 nM and 23.6 nM, respectively. The reaction mechanism of probe 1 toward H2S and N2H4 was established using 1H NMR and HR-MS spectroscopy. Additionally, probe 1 was used for monitoring and differentiating H2S and N2H4 in HeLa cells and displays good biocompatibility and low cytotoxicity.
Topics & Concepts
FluorescenceHydrogen sulfideChemistryHydrazine (antidepressant)Dual (grammatical number)Turn (biochemistry)SulfidePhotochemistryBiochemistryOrganic chemistryLiteratureSulfurQuantum mechanicsPhysicsArtSulfur Compounds in BiologyMolecular Sensors and Ion DetectionElectrochemical sensors and biosensors