Characterization of Tremulane Sesquiterpene Synthase from the Basidiomycete <i>Irpex lacteus</i>
Rong Chen, Tao Feng, Min Li, Xinyi Zhang, Juan He, Ben Hu, Zixin Deng, Tiangang Liu, Ji‐Kai Liu, Xinghuan Wang, Guangkai Bian
Abstract
Tremulane sesquiterpenoids are key secondary metabolites of the basidiomycete Irpex lacteus, which displays structural diversity and various bioactivities. However, tremulane sesquiterpene synthases have not been reported to date. The tremulane sesquiterpene synthase of I. lacteus was characterized by genome mining, heterologous expression, an in vitro assay, and substrate feeding. Moreover, the structures of the corresponding products were elucidated by NMR spectroscopy and X-ray diffraction analysis.
Topics & Concepts
SesquiterpeneChemistryATP synthaseHeterologous expressionStereochemistryHeterologousBiochemistryEnzymeGeneRecombinant DNAPlant biochemistry and biosynthesisFungal Biology and ApplicationsMicrobial Natural Products and Biosynthesis