Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine
Jia Wen, Gilberto Padilla Mercado, Alyssa Volland, Heidi L. Doden, Colin R. Lickwar, Taylor Crooks, Genta Kakiyama, Cecelia Kelly, Jordan L. Cocchiaro, Jason M. Ridlon, John F. Rawls
Abstract
bile acid that undergo multiple microbial modifications including bile acid deconjugation that augments Fxr activity. Using single-cell RNA sequencing, we provide a cellular atlas of the zebrafish intestinal epithelium and uncover roles for Fxr in transcriptional and differentiation programs in ileal and other cell types. These results establish zebrafish as a nonmammalian vertebrate model for studying bile salt metabolism and Fxr signaling.
Topics & Concepts
ZebrafishFunction (biology)Nuclear receptorMetabolismSignal transductionCell biologyBiologyReceptorComputational biologyChemistryBiochemistryGeneTranscription factorDrug Transport and Resistance MechanismsMicroRNA in disease regulationPediatric Hepatobiliary Diseases and Treatments