Surface plasmon resonance study of the interaction of <i>N</i>-methyl mesoporphyrin IX with G-quadruplex DNA
Marine Perenon, Hugues Bonnet, Thomas Lavergne, Jérôme Dejeu, Éric Defrancq
Abstract
Surface plasmon resonance (SPR) was used to investigate the interaction between N-methyl mesoporphyrin IX (NMM) and different G-quadruplex (G4) topologies. The study was associated with circular dichroism analysis (CD) to assess the topology of the G4s when they interacted with NMM. We demonstrate the high selectivity of NMM for the parallel G4 structure with a dissociation constant at least ten times lower than those of other G4 topologies. We also confirm the ability of NMM to shift the G4 conformation from both the hybrid and antiparallel topologies toward the parallel structure.
Topics & Concepts
Surface plasmon resonanceG-quadruplexResonance (particle physics)ChemistryNuclear magnetic resonanceDNAStereochemistryCrystallographyPhysicsMaterials scienceNanotechnologyNanoparticleAtomic physicsBiochemistryDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery