Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2
Tin Maršić, Zahir Ali, Muhammad Tehseen, Ahmed Mahas, Samir M. Hamdan, Magdy M. Mahfouz
Abstract
est), to reverse transcription-recombinase polymerase amplification to detect SARS-CoV2 in clinical samples. Vigilant exhibited a limit of detection of 2.5 copies/μL, comparable to CRISPR-based systems, and showed no cross-reactivity with SARS-CoV1 or MERS. Vigilant offers an easy-to-use, rapid, cost-effective, and robust detection platform for SARS-CoV2.
Topics & Concepts
Nucleic acidOligonucleotideChemistryCRISPRBiologyMolecular biologyDNABiochemistryGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesSARS-CoV-2 detection and testing