Discovery of a lagriamide polyketide by integrated genome mining, isotopic labeling, and untargeted metabolomics
Claire H. Fergusson, Julia Saulog, Bruno S. Paulo, Darryl M. Wilson, Dennis Y. Liu, Nicholas J. Morehouse, Samantha C. Waterworth, John Barkei, Christopher A. Gray, Jason C. Kwan, Alessandra S. Eustáquio, Roger G. Linington
Abstract
Whole genome sequencing of a Burkholderiales strain library led to the discovery of the antifungal polyketide lagriamide B. Combining data from the genome sequence, NMR experiments and computational predictions defined the full absolute configuration.
Topics & Concepts
PolyketideMetabolomicsComputational biologyGenomeChemistryBiologyBioinformaticsGeneticsGeneBiosynthesisMarine Sponges and Natural ProductsMicrobial Natural Products and BiosynthesisPhytochemistry and Bioactive Compounds