Structural characterization, antioxidant and cytotoxic effects of iron nanoparticles synthesized using <i>Asphodelus aestivus</i> Brot. aqueous extract
Burcu Sümer Tüzün, Tuğçe Fafal, Pelin Taştan, Bijen Kıvçak, Besra Özmen Yelken, Çağla Kayabaşı, Sunde Yılmaz Süslüer, Cumhur Gündüz
Abstract
Abstract ASP was used to synthesize FeNP A . They were characterized by UV-vis spectroscopy, FT-IR, TEM, SEM, XRD and ZP. The aim of this study was to evaluate in vitro cytotoxic activity and antioxidant acitivities of FeNP A and ASP . The antioxidant properties were evaluated using DPPH, ABTS + and H 2 O 2 assays. FeNP A had higher antioxidant activity comparing to ASP according to DPPH (IC 50 : 3.48 μg/mL) and ABTS + (60.52%) assays. Anti-cancer activities of FeNP A and ASP were investigated in breast cancer, melanoma and control cell lines. FeNP A was more cytotoxic than ASP in MCF-7, MeWo, CHL-1, and HEL 299 cells. FeNP A had shown that mitochondria induce apoptosis through stress in MDA-MB-231, and cells MeWo. ASP also induced apoptosis 2.23-fold in MCF-7 cells. Progesterone receptor gene expression showed a 10-fold increase in a hormone-dependent MCF-7 cell line in ASP , and FeNP A treatment. Expressions of BCL6, CXCL12, DNAJC15, RB1 and TPM1 in melanoma cancer cell lines were significantly increased in ASP and FeNP A administration. It had been shown that FeNP A regulates gene expressions that may be considered important in terms of prognosis in breast cancer and melanoma cell lines and it is suggested that gene expressions regulated by FeNP A are also evaluated in animal models in vivo.