Miniaturized sensor for electroanalytical and electrochemiluminescent detection of pathogens enabled through laser-induced graphene electrodes embedded in microfluidic channels
Florian Gerstl, Uma Pongkitdachoti, Fuangfa Unob, Antje J. Baeumner
Abstract
for the EC approach and furthermore enabled highly specific detection considering that at room temperature in this simple setup a single nucleotide polymorphism resulted in a signal decrease of 58%, whereas a decrease of > 98% was observed for non-matching sequences present in 10-fold excess. Direct detection in various matrices ranging from drinking water to soil extracts was also achieved. It is concluded that the simple and inexpensive fabrication in combination with signal amplification strategies makes these concepts relevant for on-site pathogen detection in resource-limited environments.
Topics & Concepts
Detection limitElectrochemiluminescenceMicrofluidicsMicrochannelAnalyteMaterials scienceNanotechnologyBiosensorAptamerChromatographyChemistryMolecular biologyBiologyAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionNanopore and Nanochannel Transport Studies