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In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies

Lionel Imbert, Rachel Lenoir-Capello, Elodie Crublet, Alicia Vallet, Rida Awad, Isabel Ayala, Céline Juillan‐Binard, Hubert Mayerhofer, Rime Kerfah, Pierre Gans, Emeric Miclet, Jérôme Boisbouvier

2020Methods in molecular biology27 citationsDOI

Topics & Concepts

ChemistryT7 RNA polymeraseProtonationNuclear magnetic resonance spectroscopyIn vitroCofactorBiochemistryMembrane proteinProtein biosynthesisMembraneAmideBiophysicsEscherichia coliEnzymeOrganic chemistryBiologyGeneBacteriophageIonRNA and protein synthesis mechanismsRNA modifications and cancerBiochemical and Molecular Research
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