Redox priming promotes Aurora A activation during mitosis
Daniel Lim, Vladimir Joukov, T.J. Rettenmaier, Akiko Kumagai, William G. Dunphy, James A. Wells, Michael B. Yaffe
Abstract
egg extracts and mammalian cells that a conserved cysteine residue within the Aurora A activation loop is crucial for Aurora A activation by autophosphorylation. We further showed that covalent disulfide adducts of this residue promote autophosphorylation of the Aurora A kinase domain. These findings reveal a potential mechanistic link between Aurora A activation and changes in the intracellular redox state during mitosis and provide insights into how novel small-molecule inhibitors may be developed to target specific subpopulations of Aurora A.
Topics & Concepts
MitosisCell biologyRedoxPriming (agriculture)ChemistryBiologyBiophysicsBotanyGerminationOrganic chemistryMicrotubule and mitosis dynamicsPhotosynthetic Processes and MechanismsMitochondrial Function and Pathology