Rapid and sensitive Cas12a-based one-step nucleic acid detection with ssDNA-modified crRNA
Qinlong Zeng, Miaojin Zhou, Zhiqing Hu, Weiheng Deng, Zhuo Li, Lingqian Wu, Desheng Liang
Topics & Concepts
Trans-activating crRNACRISPRChemistryNucleic acidRNADNAAmpliconComputational biologyBiochemistryCas9BiologyGenePolymerase chain reactionCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms