Proteome reallocation from amino acid biosynthesis to ribosomes enables yeast to grow faster in rich media
Johan Björkeroth, Kate Campbell, Carl Malina, Rosemary Yu, Francesca Di Bartolomeo, Jens Nielsen
Abstract
Significance The yeast Saccharomyces cerevisiae is a well-studied organism, which is used as a model organism for studying eukaryal biology and as a cell factory for the production of fuels, chemicals, and pharmaceuticals. For both applications, the way that the cell utilizes its finite protein resource and how those inherent trade-offs manifest themselves is of interest, not least for their impact on cellular metabolism. Here we elucidate how alterations of protein-allocation allow for S. cerevisiae to increase its growth rate. Our results on cellular proteome-allocation may aid the engineering of more efficient strains in industrial biotechnology as well as improve our understanding toward phenotypes of cancer cells that grow faster than normal cells.