DNAzyme Nanomachine with Fluorogenic Substrate Delivery Function: Advancing Sensitivity in Nucleic Acid Detection
Z. A. Hussein, Moustapha A. Y. Nour, Anastasia V. Kozlova, Dmitry M. Kolpashchikov, Andrey B. Komissarov, A.A. Eldeeb
Abstract
We have developed a hook-equipped DNA nanomachine (HDNM) for the rapid detection of specific nucleic acid sequences without a preamplification step. HDNM efficiently unwinds RNA structures and improves the detection sensitivity. Compared to the hookless system, HDNM offers an 80-fold and 13-fold enhancement in DNA and RNA detection, respectively, reducing incubation time from 3 to 1 h.
Topics & Concepts
DeoxyribozymeChemistryNucleic acidDNARNANanotechnologyNucleic acid quantitationLocked nucleic acidDetection limitSubstrate (aquarium)Molecular biologyBiophysicsBiochemistryChromatographyOligonucleotideGeneMaterials scienceOceanographyBiologyGeologyAdvanced biosensing and bioanalysis techniquesNanopore and Nanochannel Transport StudiesBiosensors and Analytical Detection