Engineered <scp>l</scp>-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments
Ana Zúñiga, Miguel Camacho Rufino, Hung‐Ju Chang, Elsa Fristot, Pauline Mayonove, El-Habib Hani, J. Bonnet
Abstract
Nissle 1917 and can detect endogenous l-lactate produced by 3D tumor spheroids with an improved dynamic range. In the future, the ALPaGA system could be used to monitor bioproduction processes and improve the specificity of engineered bacterial cancer therapies by restricting their activity to the lactate-rich microenvironment of solid tumors.
Topics & Concepts
Anoxic watersChemistryBiochemistryFood scienceEnvironmental chemistryCancer Research and Treatmentsbioluminescence and chemiluminescence research3D Printing in Biomedical Research