Altered counts and mitochondrial mass of peripheral blood leucocytes in patients with chronic hepatitis B virus infection
Ruo‐Ran Zhou, Ya‐Hui Song, Cheng‐Yu Xu, Yingying Zhang, Xiang‐Wei Wu, Lu Zhang, Xi‐Ni Luo, Han Zhao, Mingming Liu, Junchi Xu, Lin Wang, Zu‐Tao Chen, Qingzhen Han
Abstract
Abstract Hepatitis B virus (HBV) damages liver cells through abnormal immune responses. Mitochondrial metabolism is necessary for effector functions of white blood cells (WBCs). The aim was to investigate the altered counts and mitochondrial mass (MM) of WBCs by two novel indicators of mitochondrial mass, MM and percentage of low mitochondrial membrane potential, MMP low %, due to chronic HBV infection. The counts of lymphocytes, neutrophils and monocytes in the HBV infection group were in decline, especially for lymphocyte ( p = 0.034) and monocyte counts ( p = 0.003). The degraded MM ( p = 0.003) and MMP low % ( p = 0.002) of lymphocytes and MM ( p = 0.005) of monocytes suggested mitochondrial dysfunction of WBCs. HBV DNA within WBCs showed an extensive effect on mitochondria metabolic potential of lymphocytes, neutrophils and monocytes indicated by MM; hepatitis B e antigen was associated with instant mitochondrial energy supply indicated by MMP low % of neutrophils; hepatitis B surface antigen, antiviral therapy by nucleos(t)ide analogues and prolonged infection were also vital factors contributing to WBC alterations. Moreover, degraded neutrophils and monocytes could be used to monitor immune responses reflecting chronic liver fibrosis and inflammatory damage. In conclusion, MM combined with cell counts of WBCs could profoundly reflect WBC alterations for monitoring chronic HBV infection. Moreover, HBV DNA within WBCs may be a vital factor in injuring mitochondria metabolic potential.