Capturing protein droplets: label-free visualization and detection of protein liquid–liquid phase separation with an aggregation-induced emission fluorogen
Chu-Qiao Liang, Lin Wang, Yun-Yi Luo, Qianqian Li, Yanmei Li
Abstract
We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of the protein condensed phase based on the lifetime of the DroProbe was also developed.
Topics & Concepts
Liquid liquidPhase (matter)ChemistryChromatographyViscosityVisualizationLiquid phaseMaterials scienceAnalytical Chemistry (journal)Organic chemistryComputer scienceThermodynamicsPhysicsComposite materialData miningRNA Research and SplicingProtein Structure and DynamicsLipid metabolism and biosynthesis