Evaluation of Serum Cryptococcal Antigen Testing Using Two Novel Semiquantitative Lateral Flow Assays in Persons with Cryptococcal Antigenemia
Caleb P Skipper, Kiiza Kandole Tadeo, Emily Martyn, Elizabeth Nalintya, Radha Rajasingham, David B. Meya, Bosco Kafufu, Joshua Rhein, David R. Boulware
Abstract
= 2) yielded Positive results. The CryptoPS assay missed 35% (7/20) of samples with CrAg LFA titers of ≤1:20. The new semiquantitative CrAg LFAs allow rapid estimation of titer levels in easy-to-perform platforms. The CrAgSQ demonstrated better qualitative sensitivity and specificity than the CryptoPS compared to the reference standard. The exact grading of the CrAgSQ results has some subjectivity, with interreader variability; however, qualitative reads were generally concordant for both assays.
Topics & Concepts
Cryptococcal meningitisFulminantAntigenCryptococcosisImmunologyCryptococcusMedicineVirologyBiologyViral diseaseHuman immunodeficiency virus (HIV)MicrobiologyFungal Infections and StudiesAntifungal resistance and susceptibilityNail Diseases and Treatments