AIE-nanoparticle assisted ultra-deep three-photon microscopy in the <i>in vivo</i> mouse brain under 1300 nm excitation
Dongyu Li, Hequn Zhang, Lina L. Streich, Yalun Wang, Ping Lü, Ling Wang, Robert Prevedel, Jun Qian
Abstract
Here we present organic AIE nanoparticles that feature high absorption cross-section under NIR-IIa three-photon excitation, which enables ultra-deep three-photon fluorescence imaging in the <italic>in vivo</italic> mouse brain.
Topics & Concepts
Two-photon excitation microscopyExcitationIn vivoNanoparticleMaterials scienceFluorescenceMicroscopyAbsorption (acoustics)Two-photon absorptionNanotechnologyBiophysicsOptoelectronicsOpticsPhysicsBiologyLaserComposite materialQuantum mechanicsBiotechnologyNanoplatforms for cancer theranosticsLuminescence and Fluorescent MaterialsMolecular Sensors and Ion Detection