Improved tumour delivery of iron oxide nanoparticles for magnetic hyperthermia therapy of melanoma <i>via</i> ultrasound guidance and <sup>111</sup>In SPECT quantification
P. Stephen Patrick, Daniel J. Stuckey, Huachen Zhu, Tammy L. Kalber, Haadi Iftikhar, Paul Southern, Joseph C. Bear, Mark F. Lythgoe, Simon Hattersley, Quentin A. Pankhurst
Abstract
In radiolabelling and single-photon emission computed tomography (SPECT) to non-invasively quantify distribution of a clinical grade iron-oxide-based nanoparticle in a mouse model of melanoma. We show that compared to manual injection, ultrasound guided delivery together with syringe-pump-controlled infusion improves both the nanoparticle concentration within the tumour, and the accuracy of delivery - reducing off-target peri-tumoural delivery. Following AMF heating, injected melanomas shrank significantly compared to non-injected controls, validating therapeutic efficacy. Systemic off-target delivery was quantified and extrapolated to predict off-target energy absorbance within safe limits for the main sites of background accumulation. With many nanoparticle-based therapies currently in development for cancer, this image-guided delivery strategy has wide potential impact beyond the field of magnetic hyperthermia. Future use in representative patient cohorts would also be enabled by the high clinical availability of both SPECT and ultrasound imaging.