A chemical tool for blue light-inducible proximity photo-crosslinking in live cells
Pratyush Kumar Mishra, Myeong‐Gyun Kang, Hakbong Lee, Seungjoon Kim, Subin Choi, Nirmali Sharma, Cheol‐Min Park, Jaewon Ko, Changwook Lee, Jeong Kon Seo, Hyun‐Woo Rhee
Abstract
-HaloTag in HEK293T cells showed that RNA-binding proteins in stress granules were exclusively enriched in the cross-linked samples. These results tell that our method can reveal the interactome of protein of interest within a short distance in live cells.
Topics & Concepts
Blue lightChemistryMaterials scienceOptoelectronicsNanotechnologyBiophysicsBiologyPhotochromic and Fluorescence ChemistryBiotin and Related StudiesClick Chemistry and Applications