Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs
Marco Jost, Daniel A. Santos, Reuben A. Saunders, Max A. Horlbeck, John S. Hawkins, Sonia M. Scaria, Thomas M. Norman, Jeffrey A. Hussmann, Christina R. Liem, Carol A. Gross, Jonathan S. Weissman
Topics & Concepts
CRISPRSubgenomic mRNABiologyIn silicoGene expressionComputational biologyGeneGuide RNARNA interferenceRegulation of gene expressionGeneticsPhenotypeRNACas9CRISPR and Genetic EngineeringSingle-cell and spatial transcriptomicsRNA and protein synthesis mechanisms