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Improving the catalytic efficiency and substrate affinity of a novel esterase from marine Klebsiella aerogenes by random and site-directed mutation

Haofeng Gao, Runtao Zhu, Zelong Li, Wanyi Wang, Ziduo Liu, Nan Hu

2021World Journal of Microbiology and Biotechnology13 citationsDOI

Topics & Concepts

Enterobacter aerogenesEnzyme kineticsEsteraseThermostabilityMutantChemistrySubstrate (aquarium)HydrolysisEnzymeBiochemistryStereochemistryActive siteBiologyEscherichia coliEcologyGeneEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionBiofuel production and bioconversion
Improving the catalytic efficiency and substrate affinity of a novel esterase from marine Klebsiella aerogenes by random and site-directed mutation | Litcius