Cryo-EM Structures of AcrD Illuminate a Mechanism for Capturing Aminoglycosides from Its Central Cavity
Zhemin Zhang, Christopher E. Morgan, Meng Cui, Edward Yu
Abstract
Here, we report cryo-EM structures of the AcrD aminoglycoside efflux pump in the absence and presence of bound gentamicin, posing the possibility that this pump is capable of capturing aminoglycosides from the central cavity of the AcrD trimer. The results indicate that AcrD utilizes charged residues to bind and export drugs, mediating resistance to these antibiotics.
Topics & Concepts
Mechanism (biology)Computer scienceComputational biologyBiologyPhysicsQuantum mechanicsPhotosynthetic Processes and MechanismsEnzyme Structure and FunctionGlycosylation and Glycoproteins Research