<i>In vitro</i> and <i>in silico</i> β-lactamase inhibitory properties and phytochemical profile of <i>Ocimum basilicum</i> cultivated in central delta of Egypt
Nagwa A. Shoeib, Lamiaa A. Al-Madboly, Amany E. Ragab
Abstract
CONTEXT: L. (Lamiaceae), relevant to the ecological variations in some areas of Egypt and other countries, yet no research was conducted on the plant cultivated in the central delta region of Egypt. Also, no previous data reported on inhibition of β-lactamases by OBJECTIVE: extracts and the individual constituents. MATERIALS AND METHODS: identified by double disc synergy, combination disc tests and PCR. The most active oil content was further tested with a nitrocefin kit for β-lactamase inhibition and investigated by docking. RESULTS: using nitrocefin kit was 11.6 µg/mL vs. 8.1 µg/mL for clavulanic acid as a standard β-lactamase inhibitor. DISCUSSION AND CONCLUSIONS: oil and methyl cinnamate against β-lactamase-producing bacteria. The results indicate that methyl cinnamate could be a potential alternative for β-lactamase inhibition.