Red blood cell rosetting enables size-based separation of specific lymphocyte subsets from blood in a microfluidic device
Kumar Abhishek, Anto Sam Crosslee Louis Sam Titus, Mai T. P. Dinh, Anton Mukhamedshin, Chandra Mohan, Sean C. Gifford, Sergey S. Shevkoplyas
Abstract
cells but declined with increasing cell rarity. Unlike the immunodensity approach, RESIZE required neither centrifugation nor cell washing after the separation and was ∼2.5-fold faster when processing the same sample volume. The results of this study suggest that integration of the RESIZE approach for high-yield isolation of lymphocyte subsets from blood could significantly streamline the manufacturing workflow and thus have a potentially transformative impact on the cost and availability of novel cellular immunotherapies.
Topics & Concepts
CentrifugationMicrofluidicsDifferential centrifugationLymphocytePlateletCD3Lymphocyte subsetsSeparation (statistics)BiologyImmunologyChromatographyChemistryCell biologyMolecular biologyT cellAntigenNanotechnologyMaterials scienceImmune systemMathematicsCD8StatisticsMicrofluidic and Bio-sensing TechnologiesHematopoietic Stem Cell TransplantationErythrocyte Function and Pathophysiology