Multiparameter microfluidics assay of thrombus formation reveals increased sensitivity to contraction and antiplatelet agents at physiological temperature
Linda Herfs, Frauke Swieringa, Natalie J. Jooss, Mike Kozlowski, Floor Heubel-Moenen, René van Oerle, Patric Machiels, Yvonne Henskens, Johan W. M. Heemskerk
Abstract
INTRODUCTION: Current developments to assess qualitative and quantitative platelet traits in flowed whole-blood are based on microfluidic devices that mostly operate at room temperature. However, operation at physiological temperature (37 °C) may increase the assay's sensitivity, and facilitates the comparison to other platelet function tests of the diagnostic laboratory. MATERIALS AND METHODS: ). These modifications allowed rapid testing of control and patient blood samples at physiological temperature. RESULTS AND CONCLUSION: and ADP in the buildup of contracted thrombi under flow. Overall, this study underlined the advantage of multiparameter assessment of microfluidic thrombus formation in detecting an acquired platelet dysfunction, when operating at physiological temperature. This work may bring microfluidics tests closer to the diagnostic laboratory.