Structural Design and Synthesis of Bimodal PNA That Simultaneously Binds Two Complementary DNAs To Form Fused Double Duplexes
Manoj K. Gupta, Bharath Raj Madhanagopal, Dhrubajyoti Datta, Krishna N. Ganesh
Abstract
Bimodal PNAs are new PNA constructs designed to bind two different cDNA sequences synchronously to form double duplexes. They are synthesized on solid phase using sequential coupling and click reaction to introduce a second base in each monomer at Cα via alkyltriazole linker. The ternary bimodal PNA:DNA complexes show stability higher than that of individual duplexes. Bimodal PNAs are appropriate to create higher-order fused nucleic acid assemblies.
Topics & Concepts
ChemistryLinkerNucleic acidMonomerDNAPeptide nucleic acidComplementary DNADouble strandedStereochemistrySolid-phase synthesisTernary operationCombinatorial chemistryBase pairOligonucleotideCrystallographyBiochemistryPolymerPeptideOrganic chemistryGeneProgramming languageComputer scienceOperating systemAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene Delivery