XPS spectra from nucleobases, DNA, and RNA
Madeleine Ramstedt, Andrey Shchukarev
Abstract
X-ray photoelectron spectroscopy was used to analyze commercially available powders of thymine, uracil, cytosine, guanine, adenine, as well as DNA isolated from salmon testes and RNA from torula yeast. The powders were pressed onto a sample holder and analyzed as received from the manufacturer. Survey spectra and high-resolution spectra of O 1s, N 1s, C 1s of all nucleobases are reported, including a small Na 1s contamination in cytosine. Spectra of DNA and RNA are also included for comparison. Furthermore, the presented data are compared with previously published results, as well as theoretical values, and differences are discussed.
Topics & Concepts
GuanineNucleobaseUracilCytosineThymineX-ray photoelectron spectroscopyDNARNASpectral lineChemistryCrystallographyAnalytical Chemistry (journal)Nuclear chemistryNucleotideBiochemistryNuclear magnetic resonanceEnvironmental chemistryPhysicsAstronomyGeneElectron and X-Ray Spectroscopy TechniquesX-ray Spectroscopy and Fluorescence AnalysisDNA and Nucleic Acid Chemistry