Effect of Membrane Potential on Entry of Lactoferricin B-Derived 6-Residue Antimicrobial Peptide into Single Escherichia coli Cells and Lipid Vesicles
Farzana Hossain, Hideo Dohra, Masahito Yamazaki
Abstract
Bacterial cells have a membrane potential (Δφ), but the effect of Δφ on the action of cell-penetrating peptide-type antimicrobial peptides (AMPs) is not clear. Here, we investigated the effect of Δφ on the action of a fluorescent probe-labeled AMP derived from lactoferricin B, Rh-LfcinB(4–9).
Topics & Concepts
SpheroplastCalceinPeptideVesicleBiologyBiophysicsCytosolAntimicrobial peptidesBiochemistryAlexa FluorIonophoreCell membraneEscherichia coliMembraneFluorescenceEnzymeGeneQuantum mechanicsPhysicsAntimicrobial Peptides and ActivitiesLipid Membrane Structure and BehaviorRNA Interference and Gene Delivery