Targeted Detoxification of Aflatoxin B<sub>1</sub> in Edible Oil by an Enzyme–Metal Nanoreactor
Caicai Fu, Lirui Hou, Dingchun Chen, Teng Huang, Shutao Yin, Ping Ding, Qiansui Liao, Xiaolin Huang, Yonghua Xiong, Jun Ge, Xiaoyang Li
Abstract
Mycotoxin contamination is an important issue for food safety and the environment. Removing mycotoxins from food without losing nutrients and flavor components remains a challenge. In this study, a novel strategy was proposed for the targeted removal of aflatoxin B 1 (AFB 1 ) from peanut oil using an amphipathic enzyme–metal hybrid nanoreactor (PL-GOx-Fe 3 O 4 @COF) constructed with covalent organic frameworks (COFs) which can selectively adsorb AFB 1 . Due to the confined space provided by COFs and the proximity effect between GOx and Fe 3 O 4, the detoxification of AFB 1 is limited in the nanoreactor without affecting the composition and properties of the oil. The detoxification efficiency of AFB 1 in the chemoenzymatic cascade reaction catalyzed by PL-GOx-Fe 3 O 4 @COF is six times higher than that of the combination of free GOx and Fe 3 O 4 . The AFB 1 transformation product has nontoxicity to kidney and liver cells. This study provides a powerful tool for the targeted removal of mycotoxins from edible oils.