Portable cell imprinted polymer-based microfluidic sensor for bacteria detection in real water
Ali Doostmohammadi, Hongsheng Huang, Sohail Naushad, Pouya Rezai
Abstract
• Cell imprinted polymer (CIP) microfluidic sensor for bacteria detection in water. • CIP-coated microparticles synthesized and used within the microfluidic device. • Sensor limits of detection at 8 × 10 3 CFU/mL and quantification at 6 × 10 5 CFU/mL. • Sensor specificity to E. coli confirmed using non-specific Salmonella and Sarcina. • Compared to lab-based method, sensor similarly quantified bacteria in pond water. Cell-imprinted polymer (CIP) based optical biosensors have transformed point-of-care detection. However, challenges remain in their portability and detection sensitivity, time, and cost. Herein, we present an imprinted polymer-based low-cost microfluidic device integrated into a portable enclosure that enables rapid and sensitive bacteria detection in real water. A portable 3D-printed platform was custom-designed, housing all essential detection components, i.e., pumping and fluorescent imaging units and the microfluidic sensor. CIP coated magnetic microparticles (MPs) with affinity to bacteria were manipulated inside the magnetophoretic microfluidic device at an optimized flow rate of 0.01 mL/min for bacteria capturing. Fluorescent imaging pre- and post-bacteria capture facilitated quantification of fluorescence intensity changes as bacteria were trapped by the CIP-MPs. The sensor’s dose–response curve established limits of detection (LOD) and quantification (LOQ) at 8 × 10 3 and 6 × 10 5 CFU/mL, respectively, within a dynamic range of 10 3 to 10 9 CFU/mL. It specifically detected E. coli , distinguishing it from non-specific bacteria like Salmonella and Sarcina . In real pond water tests, our sensor detected 2 × 10⁶ CFU/mL, matching a central lab’s result of 2.33 × 10⁶ CFU/mL, demonstrating its effectiveness for real-water monitoring. While further enhancements are needed for improving the specificity in complex environmental matrices and broader bacterial strain detection, the sensor’s simplicity and portability highlight its potential for practical potential.