Heterologous Expression and Biochemical Analysis Reveal a Schizokinen-Based Siderophore Pathway in <i>Leptolyngbya</i> (Cyanobacteria)
Suqin Wang, Leanne A. Pearson, Rabia Mazmouz, Tianzhe Liu, Brett A. Neilan
Abstract
sp. PCC 7376, and many other cyanobacteria, use schizokinen-based iron chelators (siderophores) to scavenge iron from the environment. We have shown that these pathways are amenable to heterologous expression in E. coli, which expands the limited arsenal of known cyanobacterial siderophores and is advantageous for the downstream overproduction of relevant siderophores of ecological and industrial value.
Topics & Concepts
SiderophoreNonribosomal peptideBiologyCyanobacteriaHeterologous expressionBiochemistryGeneGene clusterActinobacteriaEscherichia coliBacteriaMicrobiologyBiosynthesisGeneticsRecombinant DNA16S ribosomal RNAMicrobial Community Ecology and PhysiologyPhotosynthetic Processes and MechanismsProtist diversity and phylogeny