A disease-driver population within interstitial cells of human calcific aortic valves identified via single-cell and proteomic profiling
Julius L. Decano, Yukio Iwamoto, Shinji Goto, Janey Y. Lee, Joan T. Matamalas, Arda Halu, Mark C. Blaser, Lang Ho Lee, Brett Pieper, Sarvesh Chelvanambi, Jessica Silva-Nicolau, Francesca Bartoli‐Leonard, Hideyuki Higashi, Haruki Shibata, Payal Vyas, Jianguo Wang, Elena V. Gostjeva, Simon C. Body, Sasha A. Singh, Masanori Aikawa, Elena Aïkawa
Abstract
and discover potential key regulators of human CAVD. These DDP-VICs demonstrate multi-lineage differentiation and osteogenic properties. Temporal proteomic profiling of DDP-VICs identifies potential targets for therapy, including MAOA and CTHRC1. In vitro loss-of-function experiments confirm our targets. Such a stepwise strategy may be advantageous for therapeutic target discovery in other disease contexts.