Modulation of Monocyte Activation and Function during Direct Antiviral Agent Treatment in Patients Coinfected with HIV and Hepatitis C Virus
Rebeca S. De Pablo-Bernal, María Reyes Jiménez‐León, Laura Tarancón‐Díez, Alicia Gutiérrez‐Valencia, Ana Serna‐Gallego, María Trujillo‐Rodríguez, Ana Isabel Álvarez‐Ríos, Yusnelkis Milanés‐Guisado, Núria Espinosa, Cristina Roca-Oporto, Pompeyo Viciana, Luís F. López‐Cortés, Ezequiel Ruíz-Mateos
Abstract
The activation phenotypes and functional changes in monocyte subsets during hepatitis C virus (HCV) elimination in HIV/HCV-coinfected patients were evaluated. Twenty-two HIV/HCV-coinfected patients on suppressive combination antiretroviral treatment (cART) achieving HCV elimination after direct-acting antiviral (DAA) therapy and 10 HIV-monoinfected patients were included. The activation phenotype (10 markers) and polyfunctionality (intracellular interleukin-1α [IL-1α], IL-1β, IL-6, IL-8, tumor necrosis factor alpha [TNF-α], and IL-10 production) in three monocyte subsets (classical, intermediate, and nonclassical) were evaluated by flow cytometry before and at the end of treatment.