Light-driven hydroxylation of testosterone by <i>Synechocystis</i> sp. PCC 6803 expressing the heterologous CYP450 monooxygenase CYP110D1
Francesco Mascia, Sara B. Pereira, Catarina C. Pacheco, Paulo Oliveira, Jennifer Solarczek, Anett Schallmey, Robert Kourist, Véronique Alphand, Paula Tamagnini
Abstract
Biotransformation of testosterone into 15β-hydroxytestosterone by the cyanobacterium Synechocystis expressing the heterologous monooxygenase CYP110D1. The reaction is sustained by reducing equivalents and oxygen provided by oxygenic photosynthesis.
Topics & Concepts
HeterologousMonooxygenaseHydroxylationBiotransformationChemistrySynechocystisCyanobacteriaBiochemistryHeterologous expressionPhotosynthesisOxygenMetabolismEnzymeBiologyCytochrome P450Recombinant DNAOrganic chemistryBacteriaGeneMutantGeneticsSteroid Chemistry and BiochemistryPhotosynthetic Processes and MechanismsPharmacogenetics and Drug Metabolism