Two-pore potassium channel TREK-1 (K<scp>2</scp>P<scp>2.1</scp>) regulates NLRP<scp>3</scp> inflammasome activity in macrophages
C.N. Immanuel, Bin Teng, Brittany Dong, Elizabeth M. Gordon, Charlean Luellen, Benjamin Lopez, Jeffrey N. Harding, Stephania A. Cormier, Elizabeth Fitzpatrick, Andreas Schwingshackl, Christopher M. Waters
2024American Journal of Physiology-Lung Cellular and Molecular Physiology18 citationsDOIOpen Access PDF
Abstract
Because of the importance of potassium efflux in inflammasome activation, we investigated the role of the two-pore potassium (K2P) channel TREK-1 in macrophage inflammasome activity. Using primary alveolar macrophages and bone marrow-derived macrophages from wild-type and TREK-1 –/– mice, we measured responses to inflammasome priming (using LPS) and activation (LPS + ATP). Our results suggest that TREK-1 is an important regulator of NLRP3 inflammasome activation in macrophages.
Topics & Concepts
InflammasomeIntracellularPotassiumPotassium channelChemistryEffluxLipopolysaccharideBiochemistryBiophysicsMolecular biologyBiologyEndocrinologyReceptorOrganic chemistryInflammasome and immune disordersAdenosine and Purinergic Signaling